U-Blot® PAKα/β/γ (phospho Thr423/402/421) Polyclonal Antibody

Name: U-Blot® PAKα/β/γ (phospho Thr423/402/421) Polyclonal Antibody
SKU: WP9992
Availability: InStock

$288.00

Size:50μL

SKU: WP9992-50

Catalog No.: WP9992

Specification: 50μL/100μL

Stock: In stock

U-Blot® PAKα/β/γ (phospho Thr423/402/421) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010],

Product Overview

Catalog No.: WP9992
SKU: WP9992-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: PAK1/2/3
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 60kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human PAK1/2/3 around the phosphorylation site of Thr423/402/421. AA range:391-440
Sequence: --
Specificity: Phospho-PAKα/β/γ (T423/402/421) Polyclonal Antibody detects endogenous levels of PAKα/β/γ protein only when phosphorylated at T423/402/421.
Gene Name: PAK1/PAK2/PAK3
Protein Name: Serine/threonine-protein kinase PAK 1/Serine/threonine-protein kinase PAK 2/Serine/threonine-protein kinase PAK 3
Other Name: PAK1;Serine/threonine-protein kinase PAK 1;Alpha-PAK;p21-activated kinase 1;PAK-1;p65-PAK;PAK2;Serine/threonine-protein kinase PAK 2;Gamma-PAK;PAK65;S6/H4 kinase;p21-activated kinase 2;PAK-2;p58;PAK3;OPHN3;Serine/threonine-p
Organism-1: Human
Gene ID-1: 5058;5062;5063
SwissProt-1: Q13153;Q13177;O75914
Organism-2: Mouse
Gene ID-2: 224105;18481
SwissProt-2: --
Organism-3: Rat
Gene ID-3: 29431;100910732;29433
SwissProt-3: P35465;Q64303;Q62829
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Cell junction, focal adhesion . Cell projection, lamellipodium . Cell membrane . Cell projection, ruffle membrane . Cell projection, invadopodium . Nucleus, nucleoplasm . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Upon DNA damage, translocates to the nucleoplasm when phosphorylated at Thr-212 where is co-recruited with MORC2 on damaged chromatin (PubMed:23260667). Localization to the centrosome does not depend upon the presence of gamma-tubulin (PubMed:27012601). Localization of the active, but not inactive, protein to the adhesions and edge of lamellipodia is mediated by interaction with GIT1 (PubMed:11896197). .