Filtered Products

Luciferase Complementation Assay (LCA) is a highly sensitive, high-throughput protein-protein interaction detection technology based on the principle of protein fragment complementation. The technique splits firefly luciferase (Fluc) or Renilla luciferase (Rluc) at specific sites (e.g., Gly437/Arg398) into an N-terminal fragment (N-Luc, ~1-437 aa) and a C-terminal fragment (C-Luc, ~438-550 aa); neither fragment alone possesses catalytic activity. When each fragment is fused to a target protein of interest (Bait and Prey, respectively), specific interaction between Bait and Prey within living cells brings the two luciferase fragments into close proximity, allowing them to reconstitute into a fully functional luciferase enzyme. In the presence of substrate (luciferin/coelenterazine), the reconstituted enzyme catalyzes the production of quantifiable bioluminescence signals. Signal intensity positively correlates with the affinity and binding efficiency of the protein interaction, enabling real-time, quantitative, and reversible detection of protein-protein interactions at the cellular level. LCA offers exceptionally low background, high signal-to-noise ratio, no requirement for exogenous substrate addition, compatibility with diverse cell types, and adaptability to high-throughput screening platforms. It is widely applied in drug target screening, signaling pathway dissection, membrane protein interaction studies, and detection of weak/transient interactions.