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RNA Immunoprecipitation (RIP) is a core molecular biology technique for studying intracellular RNA-protein interactions, serving as a powerful tool for deciphering the dynamic processes of post-transcriptional regulatory networks and revealing the functional roles of RNA-binding proteins (RBPs). RIP leverages the antigen-antibody specific recognition principle to capture and identify RNA bound by target proteins. Cells are lysed under mild conditions (containing RNase inhibitors) to maintain the native binding state of RNA-protein complexes. A specific antibody against the target RNA-binding protein or protein of interest is added to bind the protein-RNA complex; Protein A/G magnetic beads are then used to capture the antibody-antigen-RNA complex. Multiple wash steps remove non-specifically bound RNA and proteins. Proteinase K digestion of the protein components to release the RNA bound to the target protein. DNase I treatment is performed to eliminate genomic DNA contamination, followed by extraction and purification of the enriched RNA fragments. The recovered RNA is then subjected to RT-qPCR analysis for quantitative validation of candidate target RNAs, or to RIP-seq (high-throughput RNA sequencing) for systematic identification of the complete RNA repertoire bound to the target protein at the whole-transcriptome level.