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Protein tyrosine nitration is a non-enzymatic PTM triggered by reactive nitrogen species (RNS) under oxidative and inflammatory conditions. This modification results in the formation of 3-nitrotyrosine through the addition of a nitro group (-NO2) to tyrosine residues, altering protein structure and function. Tyrosine nitration is recognized as a marker of nitrosative stress and has been implicated in inflammation, aging, and chronic diseases.