- Molecule
- TNFSF5/CD154/CD40LG
- Background
- Human CD40L (CD154) is one member of TNF family which is expressed on T cells. It binds the CD40, which is on the antigen-presenting cells (ex: B cells, macrophages and monocytes) Human CD40L is a 21.6 kDa cytokine with 103 amino acid residues which is a transmembrane glycoprotein. Human CD40L plays an important role in the adaptive immune system which induces the B cell maturation and activation.
- Species
- Human
- Source
- E. coli
- Tags
- C-6His
- Accession
- P29965
- KnownAs
- soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM, sCD40 Ligand
- Protein Length
- Met1-Leu261
- Predicted Molecular Mass
- 16.99 kDa
- N-terminal Sequence
- --
- SDS-PAGE
- 16 kDa, reducing condition
- Endotoxin
- <0.1 EU/µg as determined by LAL test.
- Concentration
- --
- Purity-SDS-PAGE
- >95% as determined by SDS-PAGE.
- Purity-SEC-HPLC
- --
- Activity
- --
- Form
- Lyophilized
- Formulation
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
- Shipping
- The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
- Storage
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3-6 months.
Please minimize freeze-thaw cycles and use a manual defrost freezer.
- Reconstitution
- Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 µg/mL, and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.