U-Blot® RNA pol II Monoclonal Antibody

U-Blot® RNA pol II Monoclonal Antibody
U-Blot® RNA pol II Monoclonal Antibody
$9,999.00
Size:100µg
SKU: WE2608-100

Catalog No.: WE2608

Specification: 100µg/10µg

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background:RNA pol II ( RNA polymerase II ) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Product Overview

Catalog No.
WE2608
SKU
WE2608-100
Category
Antibodies from other sources
Product Type
Other
Size
100µg/10µg
Stock Status
In stock
Available Stock
999
Minimum Order Quantity
1

Storage & Compliance

Research Use Only
Yes

Additional Specifications

Target
--
Reactivity
Human
Applications
ChIP; ChIP-Seq; WB; IF; ICC
MW(Calculated)
240 kDa
Host Species
Rat
Isotype
IgG2a
Recommended Dilution Ratio
Applications Validated by Active Motif: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 - 10 µg each ICC/IF: 5 µg/ml dilution WB*: 0.5 - 2 µg/ml dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here . *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. For RNA Pol II , we also offer AbFlex ® RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151 .
Formulation
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Purification
Protein G Chromatography
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Concentration
--
Clonality
Monoclonal
Clone Number
1F4B6
Immunogen
This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.
Specificity
--
Gene Name
--
Other Name
RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
Gene ID
--
Cellular Localization
--
Function
--

Tech Support

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Contact Support

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Phone+(1)-866-986-9598

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Keywords:Antibodies from other sources