- Target
- STAT3
- Reactivity
- Human
- Applications
- ICFC,ICC, ChIP,
- MW(Calculated)
- --
- Host Species
- Mouse
- Isotype
- Mouse IgG1, κ
- Recommended Dilution Ratio
- Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol . For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration of 2.5 μg/mL is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Purification
- The antibody was purified by affinity chromatography.
- Storage
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Concentration
- 0.5 mg/ml
- Clonality
- Monoclonal
- Clone Number
- RMM6089
- Immunogen
- Human Stat3 peptide phosphorylated at Ser 727. Complete Freund's adjuvant.
- Specificity
- STAT3 Phospho Ser727
- Gene Name
- --
- Other Name
- Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
- Gene ID
- 6774
- Cellular Localization
- Nuclear Markers, Phospho-Proteins
- Function
- STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.