U-Blot® Crystallin-αB (phospho Ser59) Polyclonal Antibody

Name: U-Blot® Crystallin-αB (phospho Ser59) Polyclonal Antibody
SKU: WP10016
Availability: InStock

$288.00

Size:50μL

SKU: WP10016-50

Catalog No.: WP10016

Specification: 50μL/100μL

Stock: In stock

U-Blot® Crystallin-αB (phospho Ser59) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distin

Product Overview

Catalog No.: WP10016
SKU: WP10016-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Crystallin-αB
Reactivity: Human,Mouse,Rat
Application: IHC,IF,ELISA
MW(Calculated): 20kD
MW(Observed): --
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human CRYAB/Crystallin-alpha-B around the phosphorylation site of Ser59. AA range:31-80
Sequence: --
Specificity: Phospho-Crystallin-αB (S59) Polyclonal Antibody detects endogenous levels of Crystallin-αB protein only when phosphorylated at S59.
Gene Name: CRYAB
Protein Name: Alpha-crystallin B chain
Other Name: CRYAB;CRYA2;Alpha-crystallin B chain;Alpha;B;-crystallin;Heat shock protein beta-5;HspB5;Renal carcinoma antigen NY-REN-27;Rosenthal fiber component
Organism-1: Human
Gene ID-1: 1410
SwissProt-1: P02511
Organism-2: Mouse
Gene ID-2: 12955
SwissProt-2: P23927
Organism-3: Rat
Gene ID-3: 25420
SwissProt-3: P23928
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Nucleus . Secreted . Lysosome . Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (PubMed:19464326). Localizes at the Z-bands and the intercalated disk in cardiomyocytes (PubMed:28493373). Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). .