U-Blot® MAD1 (phospho Ser428) Polyclonal Antibody

Name: U-Blot® MAD1 (phospho Ser428) Polyclonal Antibody
SKU: WP10037
Availability: InStock

$288.00

Size:50μL

SKU: WP10037-50

Catalog No.: WP10037

Specification: 50μL/100μL

Stock: In stock

U-Blot® MAD1 (phospho Ser428) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],

Product Overview

Catalog No.: WP10037
SKU: WP10037-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: MAD1
Reactivity: Human,Rat,Mouse,
Application: IHC,IF,ELISA
MW(Calculated): 83kD
MW(Observed): --
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human MAD1 around the phosphorylation site of Ser428. AA range:394-443
Sequence: --
Specificity: Phospho-MAD1 (S428) Polyclonal Antibody detects endogenous levels of MAD1 protein only when phosphorylated at S428.
Gene Name: MAD1L1
Protein Name: Mitotic spindle assembly checkpoint protein MAD1
Other Name: MAD1L1;MAD1;TXBP181;Mitotic spindle assembly checkpoint protein MAD1;Mitotic arrest deficient 1-like protein 1;MAD1-like protein 1;Mitotic checkpoint MAD1 protein homolog;HsMAD1;hMAD1;Tax-binding protein 181
Organism-1: Human
Gene ID-1: 8379
SwissProt-1: Q9Y6D9
Organism-2: Mouse
Gene ID-2: --
SwissProt-2: Q9WTX8
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus . Chromosome, centromere, kinetochore . Nucleus envelope . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Co-localizes with TPR at the nucleus envelope during interphase and throughout the cell cycle (PubMed:22351768, PubMed:18981471). From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody (PubMed:9546394). Localizes to kinetochores during prometaphase (PubMed:22351768, PubMed:29162720). Does not localize to kinetochores during metaphase (PubMed:29162720). Colocalizes with NEK2 at the kinetochore (PubMed:14978040). Colocalizes with IK at spindle poles during metaphase and anaphase (PubMed:22351768). .; [Isoform 3]: Cytoplasm .