U-Blot® Integrin β1 (phospho Tyr795) Polyclonal Antibody

Name: U-Blot® Integrin β1 (phospho Tyr795) Polyclonal Antibody
SKU: WP9528
Availability: InStock

$288.00

Size:50μL

SKU: WP9528-50

Catalog No.: WP9528

Specification: 50μL/100μL

Stock: In stock

U-Blot® Integrin β1 (phospho Tyr795) Polyclonal Antibody Learn more

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Background：Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.: WP9528
SKU: WP9528-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Integrin β1
Reactivity: Human,Mouse,Rat
Application: WB,ELISA
MW(Calculated): --
MW(Observed): 140kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human ITGB1 around the phosphorylation site of Tyr795. AA range:749-798
Sequence: --
Specificity: Phospho-Integrin β1 (Y795) Polyclonal Antibody detects endogenous levels of Integrin β1 protein only when phosphorylated at Y795.
Gene Name: ITGB1
Protein Name: Integrin beta-1
Other Name: ITGB1;FNRB;MDF2;MSK12;Integrin beta-1;Fibronectin receptor subunit beta;VLA-4 subunit beta;CD antigen CD29
Organism-1: Human
Gene ID-1: 3688
SwissProt-1: P05556
Organism-2: Mouse
Gene ID-2: 16412
SwissProt-2: P09055
Organism-3: Rat
Gene ID-3: 24511
SwissProt-3: P49134
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cell membrane ; Single-pass type I membrane protein . Cell projection, invadopodium membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single-pass type I membrane protein . Recycling endosome . Melanosome . Cleavage furrow . Cell projection, lamellipodium . Cell projection, ruffle . Cell junction, focal adhesion . Cell surface . Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner. .; [Isoform 5]: Cell membrane, sarcolemma . Cell junction . In cardiac muscle, isoform 5 is found in costameres and intercalated disks. .