U-Blot® Smad3 (Phospho Ser423/Ser425) Rabbit mAb

U-Blot® Smad3 (Phospho Ser423/Ser425) Rabbit mAb
U-Blot® Smad3 (Phospho Ser423/Ser425) Rabbit mAb
$288.00
Size:50μL
SKU: WR4635-50

Catalog No.: WR4635

Specification: 50μL/100μL

Stock: In stock

U-Blot® Smad3 (Phospho Ser423/Ser425) Rabbit mAb Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

Product Overview

Catalog No.
WR4635
SKU
WR4635-50
Category
Rabbit mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
Smad3
Reactivity
Human,Mouse
Application
WB,IHC,IF,ELISA
MW(Calculated)
48kD
MW(Observed)
52kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Phospho
Modification site
--
Recommended Dilution Ratio
IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
Form
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
--
Purification
Protein A
Purity
--
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
--
Clone No.
PT0913R
Immunogen
--
Sequence
--
Specificity
Phospho-Smad3 (S423/S425) Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S423/425.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):CSSVs
Gene Name
SMAD3
Protein Name
Mothers against decapentaplegic homolog 3
Other Name
SMAD3;MADH3;Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3
Organism-1
Human
Gene ID-1
4088
SwissProt-1
P84022
Organism-2
Mouse
Gene ID-2
17127
SwissProt-2
Q8BUN5
Organism-3
Rat
Gene ID-3
25631
SwissProt-3
P84025
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .

Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

Emailinfo@ucallmlabs.com

Phone+(1)-866-986-9598

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Keywords:Rabbit mAbs