U-Blot® Rb (Phospho Ser807/811) Rabbit mAb

U-Blot® Rb (Phospho Ser807/811) Rabbit mAb
U-Blot® Rb (Phospho Ser807/811) Rabbit mAb
$288.00
Size:50μL
SKU: WR4806-50

Catalog No.: WR4806

Specification: 50μL/100μL

Stock: In stock

U-Blot® Rb (Phospho Ser807/811) Rabbit mAb Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.
WR4806
SKU
WR4806-50
Category
Rabbit mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
Rb
Reactivity
Human,Mouse,Rat
Application
WB,IHC,IF,ELISA
MW(Calculated)
106kD
MW(Observed)
106kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Phospho
Modification site
Ser807/811
Recommended Dilution Ratio
IHC 1:200-1:1000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
Form
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
--
Purification
Protein A
Purity
--
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
Monoclonal
Clone No.
PT0996R
Immunogen
--
Sequence
--
Specificity
Rb (Phospho Ser807/811) Antibody detects endogenous levels of Rb protein only when phosphorylated at S807.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):YIsPL/LKsPY
Gene Name
RB1
Protein Name
Retinoblastoma-associated protein
Other Name
RB1;Retinoblastoma-associated protein;p105-Rb;pRb;Rb;pp110
Organism-1
Human
Gene ID-1
5925
SwissProt-1
P06400
Organism-2
Mouse
Gene ID-2
19645
SwissProt-2
P13405
Organism-3
Rat
Gene ID-3
24708
SwissProt-3
P33568
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
Disease:Defects in RB1 are a cause of bladder cancer [MIM:109800].,Disease:Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].,Disease:Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated.,Function:Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.,online information:RB1 mutation db,online information:Retinoblastoma protein entry,PTM:Phosphorylated in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb.,similarity:Belongs to the retinoblastoma protein (RB) family.,subunit:Interacts with ATAD5 (By similarity). The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor. The unphosphorylated form interacts with ARID3B, KDM5A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with NDC80. Interacts with EID1 and UBR4. Interacts with ARID4A and KDM5B. Interacts with E4F1. Interacts with adenovirus E1A protein, HPV E7 protein and SV40 large T antigen.,tissue specificity:Expressed in the retina.,

Tech Support

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

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Keywords:Rabbit mAbs