U-Blot® Human Nitrated Alpha-synuclein (Tyr125/133) Quantitative ELISA Kit

U-Blot® Human Nitrated Alpha-synuclein (Tyr125/133) Quantitative ELISA Kit
U-Blot® Human Nitrated Alpha-synuclein (Tyr125/133) Quantitative ELISA Kit
$9,999.00
SKU: WT131E

Specification: 1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Product details, specifications, documents, and related products are available from the catalog record.

Product Overview

Catalog No.
WT131E
SKU
WT131E
Category
Nitration
Product Type
Other
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-20°C
Research Use Only
Yes

Additional Specifications

Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species
Human
Compatible Sample Types
Tissue Lysates, Cell Lysates
Accession Number
P37840
Gene Id
6622
Gene Symbols
SNCA NACP PARK1
Protein Name / Synonyms
Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP)
Solid Support
96-well Microplate
Design Principle
Sandwich-based
Method Of Detection
Colorimetric
Research Area
Neuroscience, Post-Translational Modifications, Inflammation, Autophagy, Nitration, Parkinson's Disease, Aging
Modification
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Shipping Type
2‑8 ℃
Storage
-20°C
Kit Components
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Additional Materials Required
Microplate reader capable of measuring absorbance at 450 nm;Protease and Phosphatase inhibitors;Precision pipettes to deliver 2 µL to 1 mL volumes;Adjustable 1-25 mL pipettes for reagent preparation;100 mL and 1 liter graduated cylinders;Log-log graph paper or computer and software for ELISA data analysis;Absorbent paper;Tubes to prepare positive control or sample dilutions;Distilled or deionized water
Assay Procedure Summary
Prepare all reagents, samples, and Positive Control as instructed. in the manual; Add 100 µL Standard to each well; Incubate 2.5 hours at room temperature or overnight at 4ºC with gentle shaking; Add 100 µL prepared detection antibody to each well; Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL prepared HRP-conjugated anti-rabbit IgG concentrate solution; Incubate for 1 hour at room temperature with gentle shaking; Add 100 µL TMB One-Step Substrate Reagent to each well; Incubate 30 minutes at room temperature; Add 50 µL Stop Solution to each well; Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
-20°C
06

Contact Support

Emailinfo@mail.ucallm.com

Phone+(1)-866-986-9598

WeChat / IMUcallm-Tech

HoursMonday-Friday 09:00-18:00 CST

Request Technical Support
Keywords:Nitration