- Size
- 56 Sample Kit
- Species
- --
- Compatible Sample Types
- Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
- Quantitative/Semi-Quantitative
- Semi-Quantitative
- Number of Targets Detected
- 70
- Solid Support
- Glass Slide
- Design Principle
- Sandwich-based
- Method Of Detection
- Fluorescence Laser Scanner
- Research Area
- Post-Translational Modifications, Glycosylation
- Introduction
- The lectin array uses standard glass slides each spotted with 14 wells of identical lectin arrays. Each lectin, together with the positive controls is arrayed in duplicate. The slides each come with a 16-well removable gasket which allows for the process of 14 samples using one slide. Four slide slides can be nested into a tray, which matches a standard microplate and allows for automated robotic high throughput process of 56 arrays simultaneously. The lectin array provides a powerful new tool for glycosylation determination, drug discovery and biomarker development; all with limited samples volumes required.
- Product Features
- High sensitivity and specificity;Low sample volume (10-100 µl per array);Large dynamic range of detection;Compatible with most sample types;Test 4 or 8 samples on each slide;Suitable for high-throughput assays
- Suggested Application
- Identify and profile the glycans in their samples;Determine whether their biomarker of interest has glycan moieties;Find specific glycan binding ligands in biological samples
- Kit Components
- Dialysis Vials;Labeling Reagent;Labeling Buffer;Stop Solution;Lectin Array Glass Slide Assembly;Sample Diluent;20X Wash Buffer I;20X Wash Buffer II;Cy3 equivalent dye‑conjugated Streptavidin;Slide Washer/Dryer;Adhesive device sealer;Floating Dialysis Rack;Manual
- Other Materials Required
- Detection antibodies of interest (For sandwich‑based method only);Orbital shaker;Laser scanner for fluorescence detection;Aluminum foil;1.5ml Polypropylene microcentrifuge tubes;KCl, NaCl, KH₂PO₄ and Na₂HPO₄ (For label‑based method only);Plastic or glass containers, beaker, stir plate and stir bar;Pipettors, pipette tips, ddH₂O and other common lab consumables
- Protocol Outline
- Dry the glass slide;Block array surfaces;Incubate samples (samples need to be biotinylated for the label‑based approach);For the sandwich‑based principle; incubate with a detection antibody cocktail;For the label‑based principle; incubate the labeled‑streptavidin;Incubate with Cy3 Equivalent Dye‑Streptavidin;Disassemble the glass slide;Scan with a gene microarray laser scanner;Perform densitometry and analysis
- Storage/Stability
- Upon receipt, all components of the Lectin Array 70 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.