Spatial Cell Atlas

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Spatial Cell Atlas

Building a Molecular Atlas of the Nervous System Decoding Cellular Diversity, State and Spatial Organization at Molecular Resolution

The nervous system comprises diverse neuronal and glial populations organized into highly specialized cellular networks that support information processing, homeostasis, plasticity, and responses to disease.

Understanding this complexity requires molecularly resolved characterization of cells across multiple biological dimensions:

  • Cellular identity - defining distinct neuronal and glial populations
  • Developmental state - tracking neural lineage progression and maturation
  • Functional state - revealing activity, stress, and disease-associated transitions
  • Spatial organization - mapping cellular distribution and interactions within brain regions

From Cell Identity to Functional State, Spatial Organization, and Cellular Interactions - build a molecularly resolved view of the nervous system.

1. Neural Cell Atlas Antibody Panel

From Cell Identity to Functional State Characterization

Integrated antibody solutions for neural cell identity, lineage, subtype, state, function, and disease-associated phenotypes.

A comprehensive neural cell profiling strategy combines validated molecular markers with multiplex imaging and spatial analysis to resolve cellular diversity, phenotypic states, tissue organization, and functional changes across the nervous system.

2. Integrated Neural Cell Profiling Strategy

Decoding Brain Complexity Across Multiple Biological Dimensions

Research DimensionBiological QuestionMolecular ReadoutRepresentative Targets
1. Cellular IdentityWhich neural cell types and subtypes are present?Define neuronal and glial composition through cell-type specific markersNeurons: NeuN, MAP2, Tubb3, CUX1, SATB2, CTIP2
Astrocytes: GFAP, ALDH11L1, S100B
Microglia: IBA1, TMEM119, P2RY12
Oligodendrocytes: OLIG2, SOX10, MBP
2. Cellular State & PhenotypeHow do cells respond, adapt, or become dysfunctional?Characterize activation, stress, aging, inflammatory and pathological statesMicroglia activation: C1q, ApoE, CD68, TREM2
Astrocyte reactivity: GFAP, AQP4, S100B
Neuronal stress: c-Fos, ATF3, HIF-1α
Degeneration: pTau, Aβ, TDP43
3. Cell-Cell CommunicationHow do neural cells interact and regulate circuits?Analyze synaptic connectivity, neurotransmission and signaling networksSynapse: PSD95, Synaptophysin, Homer1, Gephyrin
Neurotransmission: vGLUT1/2, GAD67, ChAT, TH
Cell signaling: BDNF, CREB, mTOR
4. Spatial Organization & ArchitectureWhere are specific cell populations located within neural circuits?Map cellular distribution, tissue organization and microenvironment nichesSpatial IF / Multiplex Imaging: Cortical layers: CUX1, SATB2, CTIP2, TLE4
Brain regions: Hypothalamic peptides, hippocampal markers Cell neighborhood profiling
5. Functional Network OutputHow do molecular changes influence neural activity and behavior?Link molecular signatures to neuronal function and circuit activityActivity markers: c-Fos, Arc, EGR1
Plasticity: CREB, CaMKII, BDNF
Pathway analysis: MAPK, PI3K/AKT, mTOR

 

We provide validated antibody solutions for resolving neural cell identity, cellular states, cell-cell interactions, spatial organization, and functional activity — enabling molecularly resolved characterization of brain structure, cellular networks, and disease-associated changes.

Immunofluorescence staining of mouse brain coronal section.
Fluorescence TargetProduct NameCatalog No.ReactivityApplicationsBiological Interpretation
NeuN (RBFOX 3) 488 nmU-Blot® NeuN Rabbit mAbWR4580Human, Mouse, RatWB, IHC, IF, ELISAIdentifies mature neurons and evaluates neuronal survival and neurodegeneration after hemorrhagic injury.
GFAP 594 nmU-Blot® GFAP Rabbit mAbWR4151Human, Mouse, RatWB, IHC, IF, IP, ELISADetects reactive astrocytes and measures astrogliosis surrounding the hematoma and injured tissue.
IBA1 440 nmU-Blot® Iba1 Rabbit mAbWR4144Human, Mouse, RatWB, IHC, IF, IP, ELISAAssesses microglial activation, neuroinflammatory responses, and immune-cell accumulation after ICH.
CD31 555 nmU-Blot® CD31 Rabbit mAbWR4185Human, Mouse, RatWB, IHC, IF, ELISALabels cerebral blood vessels to evaluate vascular integrity, angiogenesis, and blood-brain barrier alterations.
MAP2 647 nmU-Blot® MAP2 Rabbit mAbWR5051Human, Mouse, RatWB, IHC, IF, ELISADetects dendritic architecture and assesses dendritic degeneration following ischemic injury.

3. Featured Products

Catalog No.Product NameReactivityValidated Applications
WR4811U-Blot® SOX-2 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR4797U-Blot® Musashi-1 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR4399U-Blot® PAX6 Rabbit mAbHuman, MouseWB, IHC, IF, ELISA
WR4338U-Blot® β3 Tubulin Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR4750U-Blot® LHFLP5 Rabbit mAbHuman, Mouse, RatWB
WR4578U-Blot® MAP2 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR8094U-Blot® PGP 9.5 Rabbit mAb (Ready to Use)Human, Mouse, RatIHC
WR4197U-Blot® PGP9.5 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR4916U-Blot® Neurofilament (NF-L) Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR8087U-Blot® Neurofilament(NF-L) Rabbit mAb (Ready to Use)HumanIHC
WR5210U-Blot® NF-M (Phospho Ser614/Ser619) Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR4138U-Blot® Neurofilament heavy polypeptide Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR5113U-Blot® HuD/HuC Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR4475U-Blot® TBR1 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR4503U-Blot® SATB2 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR4403U-Blot® Ctip2 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR5171U-Blot® FoxP2 Rabbit mAbHuman, Mouse, RatWB, IF, IP, ELISA
WR4938U-Blot® FoxA2 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, IP, ELISA
WR8089U-Blot® OLIG2 Rabbit mAb (Ready to Use)Human, MouseIHC
WR4617U-Blot® OLIG2 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR4425U-Blot® SOX10 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA, IP
WR8105U-Blot® SOX9 Rabbit mAb (Ready to Use)Human, Mouse, Rat, BovineIHC
WR4149U-Blot® SOX9 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR5369U-Blot® MYT1 Rabbit mAbHuman, Mouse, RatWB, IHC, IF, ELISA
WR8119U-Blot® TTF-1 Rabbit mAb (Ready)Human, Mouse, RatIHC

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